SIVARAMAKRISHNAN et al: α-AMYLASE PRODUCTION BY A. ORYZAE USING SSF
نویسندگان
چکیده
This study presents production of α-amylase by Aspergillus oryzae in solid-state fermentation using 14 agro-industrial wastes as substrate. Enzyme production was growth associated and maximum titers (15095 U/gds) were obtained after 72 h when incubated at 30°C on wheat bran (initial moisture content, 60%; initial medium pH, 5). Enzyme titers increased significantly when the solid medium was supplemented with additional N (sodium nitrate) and C (starch) sources.
منابع مشابه
Evaluation of Ca-Independent a-Amylase Production by Bacillus sp. KR-8104 in Submerged and Solid State Fermentation Systems
This study investigates the production of crude Ca-independent and low pH active α-amylase by Bacillussp. KR-8104 in submerged fermentation (SmF) and solid-state fermentation (SSF) systems. Differentparameters were evaluated in each system using “one factor at a time” approach to improve the production ofenzyme. The results showed that in the SmF the maximum enzyme production ...
متن کاملAmylase production from Aspergillus oryzae LS1 by solid-state fermentation and its use for the hydrolysis of wheat flour
Nine Aspergillus and three of Trichoderma strains were grown on wheat bran (WB) medium under solid state fermentation (SSF) for amylase production. Aspergillus oryzae LS1 produced the highest level of the enzyme. The thermal stability profile of its crude enzyme revealed the half-life time of more than 2 h at 50 and 60ºC. The enzyme production was affected by strain type, incubation periods, le...
متن کاملPartial purification and characterization of α-amylase produced by Aspergillus oryzae using spent-brewing grains
Solid-state fermentation (SSF) was carried out to produce α-amylase from Aspergillus oryzae (IFO 30103) using spent brewing grains (SBG) as substrate. A maximum of 11296 U/gds amylase activity was obtained after 48 h of fermentation. The extracted enzyme was subjected to partial purification by ammonium sulphate fractionation. Maximum specific activity was obtained with 40-70% fraction. SDS-PAG...
متن کاملMolecular Analysis and Improvement of Protein Production by Aspergillus oryzae Grown on Solid Substrates
Aspergillus oryzae grown in submerged and solid state fermentations revealed a number of differences in molecular and physiological aspects that are correlated with the different growth conditions. First, the aerial hyphae that occur only in solid state fermentation are mainly responsible for oxygen uptake. Second, solid state fermentation is characterised by gradients in temperature, water act...
متن کاملProduction of α-Amylase by Aspergillus terreus NCFT 4269.10 Using Pearl Millet and Its Structural Characterization
In this investigation, Aspergillus terreus NCFT4269.10 was employed in liquid static surface (LSSF) and solid state (SSF) fermentation to assess the optimal conditions for α-amylase biosynthesis. One-variable-at-a-time approach (quasi-optimum protocol) was primarily used to investigate the effect of each parameter on production of amylase. The maximum amylase production was achieved using pearl...
متن کامل